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Immunology and Infection

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JoVE Journal Immunology and Infection
Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages
 

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Article DOI: 10.3791/50323-v • 10:07 min • June 23rd, 2013
June 23rd, 2013 •
1, 2, 1, 3, 3
1Department of Animal Science, Texas A&M University, 2Department of Biology, Texas A&M University, 3Department of Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University

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The article describes a readily easy adaptive in vitro model to investigate macrophage polarization. In the presence of GM-CSF/M-CSF, hematopoietic stem/progenitor cells from the bone marrow are directed into monocytic differentiation, followed by M1 or M2 stimulation. The activation status can be tracked by changes in cell surface antigens, gene expression and cell signaling pathways.

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Keywords: Macrophage Polarization Bone Marrow Derived Macrophages GM-CSF M-CSF Monocytic Differentiation M1 Polarization M2 Polarization Cell Surface Antigens Gene Expression Cell Signaling Pathways
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