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Utvikling, utvidelse, og<em> In vivo</em> Overvåking of Human NK-celler fra humane embryonale stamceller (hESCs) og og Induced pluripotent stamceller (iPSCs)
JoVE Journal
Bioingegneria
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JoVE Journal Bioingegneria
Development, Expansion, and In vivo Monitoring of Human NK Cells from Human Embryonic Stem Cells (hESCs) and Induced Pluripotent Stem Cells (iPSCs)
DOI:

09:02 min

April 23, 2013

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Capitoli

  • 00:05Titolo
  • 01:18Setting up TrypLE-passaged ES Cells into Stage I Spin Embryoid Bodies (EBs): Day of Spin EB Setup into Stage I Differentiation (Day 0)
  • 03:16Development, Expansion, and Characterization of NK Cells from hPSC-derived Progenitor Cells
  • 04:19In vivo Fluorescent and Bioluminescent Imaging to Monitor hESC-derived NK Cells and Tumor Cells in Immunodeficient Mice
  • 06:50Results: The Generation of Hematopoietic Progenitor Cells Using the Spin EB Approach Allows for Optimal NK Cell Development from hESCs and iPSCs
  • 08:43Conclusion

Summary

Traduzione automatica

Denne protokollen beskriver utviklingen, utvidelser og in vivo avbildning av NK-celler avledet fra hESCs og iPSCs.

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