È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 01:37Assembly of Recombinant Core Histones into Octamers
- 03:14Purification of Histone Octamers by Size Exclusion Chromatography |
- 05:32Sedimentation Velocity Analysis of Reconstituted Nucleosomal Arrays
- 07:50Results: Nucleosomal Arrays from Recombinant Xenopus Core Histones
- 09:56Conclusion
A method is presented for the reconstitution of model nucleosomal arrays from recombinant core histones and tandemly repeated nucleosome positioning DNA. We also describe how sedimentation velocity experiments in the analytical ultracentrifuge, and atomic force microscopy (AFM) are used to monitor the extent of nucleosomal array saturation after reconstitution.
