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A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination
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Biologia
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JoVE Journal Biologia
A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination

A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination

DOI:
10.3791/50374-v

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12:33 min

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June 25, 2014

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, , ,
1Department of Nutrional Sciences,University of Wisconsin-Madison, 2Department of Medicine, Division of Endocrinology, Diabetes, and Metabolism,University of Wisconsin-Madison, 3School of Pharmacy,University of Waterloo

Capitoli

  • 00:05Titolo
  • 01:13Inflating the Pancreas
  • 03:47Pancreas Removal
  • 04:57Washing
  • 08:17Picking Islets
  • 09:16cAMP Assay
  • 11:19Results: Secreted Insulin can be Measured from the cAMP Stimulation Medium
  • 12:09Conclusion

Summary

Traduzione automatica

Traduzione automatica

Assaying in vitro β-cell function using isolated mouse islets of Langerhans is an important component in the study of diabetes pathophysiology and therapeutics. While many downstream applications are available, this protocol specifically describes the measurement of intracellular cyclic adenosine monophosphate (cAMP) as an essential parameter determining β-cell function.

Tags

Pancreatic Islet IsolationIntracellular CAMP DeterminationDiabetes PathophysiologyBeta-cell FunctionIncretin ReceptorsType 1 DiabetesType 2 Diabetes

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