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Immunologia e infezioni

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小鼠牙龈细胞的分离，处理和分析

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Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Isolation, Processing and Analysis of Murine Gingival Cells

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小鼠牙龈细胞的分离，处理和分析

DOI:

10.3791/50388-v

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09:47 min

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July 02, 2013

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Gabriel Mizraji*^1,2, Hadas Segev*¹, Asaf Wilensky*², Avi-Hai Hovav*¹

¹Institute of Dental Sciences,Hebrew University – Hadassah Medical Center, ²Department of Periodontology,Hebrew University – Hadassah Medical Center

Capitoli

00:05Titolo
01:49Upper Gingival Excision
03:24Gingival Processing
04:57Extracellular and Intracellular Antibody Staining for Flow Cytometry Analysis
06:58Representative Gingival Cell Analysis
08:40Conclusion

Summary

Traduzione automatica

本研究中描述了一种有效的技术，用于隔离和处理，以产生一个单一的细胞培养的小鼠口腔牙龈组织。得到的细胞可进一步用于流式细胞分析和分子水平的研究。

Tags

Murine Gingival Cells Isolation Flow Cytometry Molecular Studies Immune Mechanisms Periodontal Diseases Alveolar Bone Langerhans Cells T Regulatory Cells Oral Immune System

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