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Immunologia e infezioni

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加速1型糖尿病感应在小鼠体内的过继转移致糖尿病的CD4 + T细胞

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Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells

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加速1型糖尿病感应在小鼠体内的过继转移致糖尿病的CD4 + T细胞

DOI:

10.3791/50389-v

•

06:27 min

•

May 06, 2013

•

Gregory Berry, Hanspeter Waldner

¹Department of Microbiology & Immunology,Pennsylvania State University College of Medicine

Capitoli

00:05Titolo
01:11Isolation of T Cells from Spleen and Lymph Nodes of BDC2.5 Mice
02:52Fluorescence-activated Cell Sorting of Diabetogenic CD4+ T Cells from BDC2.5 Mice
04:28Adoptive Transfer of Diabetogenic CD4+ T Cells from BDC2.5 Mice
05:12Monitoring Recipient Mice for Hyperglycemia and T1D
05:36Results: Adoptive T-cell Transfer
06:10Conclusion

Summary

Traduzione automatica

我们提供了一个可重复的方法，诱导1型糖尿病（T1D）在小鼠胰岛抗原特异性的，初级的CD4 + T细胞过继转移在两个星期内。

Tags

Accelerated Type 1 Diabetes Adoptive Transfer Diabetogenic CD4+ T Cells NOD Mouse Autoimmune Diabetes T Cell Receptor BDC2.5 NOD.SCID Recipient T1D Pathogenesis Therapeutic Interventions

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