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Capitoli
- 00:05Titolo
- 02:40Amplification of the Template
- 04:01Vacuum Workstation Protocol
- 04:05Immobilizing the Biotinylated PCR Products
- 05:06Denaturation of DNA and Addition of Sequencing Primer
- 07:30Annealing Sequencing Primer to DNA Strands
- 07:55Pyromark Q24 Operation for Pyrosequencing
- 07:58Preparing for a Run
- 08:58Starting a Run
- 09:46Completing a Run
- 10:03Results: Pyrosequencing Identifies Bacteria and Detects Antibiotic Drug Resistance
- 11:35Conclusion
Pyrosequencing é uma técnica versátil, que facilita a sequenciação do genoma microbiano que pode ser utilizado para identificar espécies bacterianas, discriminar estirpes bacterianas, e detectar mutações genéticas que conferem resistência a agentes anti-microbianos. Neste vídeo, o procedimento para a geração microbiana amplicon, amplicon pyrosequencing, e análise da seqüência do DNA será demonstrado.
Tags
Keywords:
PyrosequencingMicrobial IdentificationMicrobial CharacterizationBacterial Species IdentificationBacterial Strain DiscriminationAntimicrobial Resistance DetectionHigh-throughput ScreeningMicrobial Genome SequencingSingle Base SequencingChemiluminescent DetectionReal-time SequencingSNP TypingMutation Detection
