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Generation of Human Cardiomyocytes: A Differentiation Protocol from Feeder-free Human Induced Pluripotent Stem Cells
JoVE Journal
Biologia
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JoVE Journal Biologia
Generation of Human Cardiomyocytes: A Differentiation Protocol from Feeder-free Human Induced Pluripotent Stem Cells
DOI:

13:18 min

June 28, 2013

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Capitoli

  • 00:05Titolo
  • 02:15Feeder-free Maintenance and Passaging of Human iPS Cell Lines
  • 03:54Embryoid Bodies Aggregation and Cardiac Induction
  • 06:25Beating Areas Isolation and Culture
  • 07:48Single Beating Cells Isolation and Analysis
  • 10:06Characterizing Contracting Cardiomyocytes Derived from iPS Cells
  • 12:36Conclusion

Summary

Traduzione automatica

Pluripotent stem cells, either embryonic or induced pluripotent stem (iPS) cells, constitute a valuable source of human differentiated cells, including cardiomyocytes. Here, we will focus on cardiac induction of iPS cells, showing how to use them to obtain functional human cardiomyocytes through an embryoid bodies-based protocol.

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