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Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
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Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)

DOI:
10.3791/50433-v

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11:00 min

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May 20, 2013

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, , , ,
1Centers for Cancer Pharmacology and Excellence in Environmental Toxicology, Department of Pharmacology,University of Pennsylvania

Capitoli

  • 00:05Titolo
  • 01:20Sample Extraction from Cells
  • 02:34Re-suspension and Filtration of Samples for UPLC
  • 03:49UPLC and Mass Spectrometer Setup
  • 06:37Differential Analysis
  • 08:47Results: Analysis of Metabolites Using UPLC-HRMS
  • 10:42Conclusion

Summary

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Untargeted metabolomics provides a hypothesis generating snapshot of a metabolic profile. This protocol will demonstrate the extraction and analysis of metabolites from cells, serum, or tissue. A range of metabolites are surveyed using liquid-liquid phase extraction, microflow ultraperformance liquid chromatography/high-resolution mass spectrometry (UPLC-HRMS) coupled to differential analysis software.

Tags

Keywords: Untargeted MetabolomicsUPLC-HRMSLiquid-liquid ExtractionPolar And Non-polar MetabolitesMicroflow UPLCPositive And Negative IonizationLabel-free Differential AnalysisMetabolic Pathway ScreeningBiomarker DiscoveryDrug Development

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