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Journal / Biology / Production of Xenopus tropicalis Egg Extracts to…
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Biology

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Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs
 

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

Article DOI: 10.3791/50434-v • 10:02 min • June 27th, 2013
June 27th, 2013 •
1,2, 1,2
1Department of Molecular Biology, Massachusetts General Hospital, 2Department of Genetics, Harvard Medical School

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We describe the collection of unfertilized Xenopus tropicalis eggs and production of a meiosis II-arrested egg extract. This egg extract can be used to purify microtubules and microtubule-associated RNAs.

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Keywords: Xenopus Tropicalis Egg Extracts Microtubule-associated RNAs Identification High-throughput Sequencing Subcellular Structures RNA Localization Xenopus Laevis
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