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重组脂膜的结构和功能研究的钾离子通道
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Biologia
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JoVE Journal Biologia
Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies
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重组脂膜的结构和功能研究的钾离子通道

DOI:
10.3791/50436-v

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10:22 min

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July 13, 2013

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, , ,
1Department of Cell Biology,University of Texas Southwestern Medical Center at Dallas

Capitoli

  • 00:05Titolo
  • 01:27Liposome Preparation and Detergent-induced Fusion of Vesicles
  • 04:15Removal of the Detergents to Form Proteoliposomes
  • 06:28Functional Study of the Ion Channel Activities in Black Lipid Membranes
  • 08:49Results: Two-Dimensional Crystallization of KvAP in Membranes
  • 09:56Conclusion

Summary

Traduzione automatica

Traduzione automatica

完整的原型脂膜的电压门控钾离子通道进入重组程序中描述的。重组的渠道都适合生化检测，电气录音，配体筛选和电子晶体学研究。这些方法可以有其它膜蛋白的结构和功能研究的一般应用。

Tags

Keywords: Kv ChannelLipid ReconstitutionMembrane ProteinsVoltage-gated Potassium ChannelLipid-protein InteractionMembrane SystemsDetergent-induced Fusion2D Crystals

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