Rivista
/
Biologia
/
Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells
JoVE Journal
Biologia
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells

Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells

DOI:
10.3791/50443-v

•

10:46 min

•

July 16, 2013

•

, ,
1Department of Cellular and Molecular Medicine, Laboratory of Molecular and Cellular Signaling,KU Leuven

Capitoli

  • 00:05Titolo
  • 02:38Isolation of Corneal Endothelial Cells
  • 04:56Culture of Bovine Corneal Endothelial Cells
  • 05:56Mechanical Stimulation for Inducing Calcium Wave
  • 07:44Results: Calcium Wave Propagation in Bovine Corneal Endothelial Cells
  • 09:39Conclusion

Summary

Traduzione automatica

Traduzione automatica

Intercellular Ca2+-waves are driven by gap junction channels and hemichannels. Here, we describe a method to measure intercellular Ca2+-waves in cell monolayers in response to a local single-cell mechanical stimulus and its application to investigate the properties and regulation of gap junction channels and hemichannels.

Tags

Keywords: Intercellular CommunicationCalcium Wave PropagationMechanical StimulationCorneal Endothelial CellsGap Junction ChannelsHemichannelsATP ReleaseCx43Fluo4-AMMicromanipulator

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/50443?article_type=v

Propagation of Human Embryonic Stem (ES) Cells
check_url/it/50443?article_type=v

Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells
check_url/it/50443?article_type=v

Study of the Actin Cytoskeleton in Live Endothelial Cells Expressing GFP-Actin
check_url/it/50443?article_type=v

Assessment of Calcium Sparks in Intact Skeletal Muscle Fibers
check_url/it/50443?article_type=v

Measuring the Osmotic Water Permeability Coefficient (Pf) of Spherical Cells: Isolated Plant Protoplasts as an Example
check_url/it/50443?article_type=v

Isolation of Murine Valve Endothelial Cells
check_url/it/50443?article_type=v

Quantitation of Endothelial Cell Adhesiveness In Vitro
check_url/it/50443?article_type=v

Isolation and Primary Culture of Mouse Aortic Endothelial Cells
check_url/it/50443?article_type=v

High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells
check_url/it/50443?article_type=v

A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions

Read Article