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Whole Cell Patch Clamp for Investigating the Mechanisms of Infrared Neural Stimulation
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JoVE Journal Neuroscienze
Whole Cell Patch Clamp for Investigating the Mechanisms of Infrared Neural Stimulation

Whole Cell Patch Clamp for Investigating the Mechanisms of Infrared Neural Stimulation

DOI:
10.3791/50444-v

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08:58 min

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July 31, 2013

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, , ,
1Biotactical Engineering, Faculty of Engineering and Industrial Science,Swinburne University of Technology, 2Department of Otolaryngology,The University of Melbourne

Capitoli

  • 00:05Titolo
  • 01:24Preparation for Patch Clamp Recordings
  • 03:12Patch Clamp Recordings for Investigation of INS
  • 04:58Making Whole Cell Recordings
  • 06:46INS Experiments
  • 07:32Spiral Ganglion Neuron Responses to INS
  • 08:21Conclusion

Summary

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Infrared nerve stimulation has been proposed as an alternative to electrical stimulation in a range of nerve types, including those associated with the auditory system. This protocol describes a patch clamp method for studying the mechanism of infrared nerve stimulation in a culture of primary auditory neurons.

Tags

Whole Cell Patch ClampInfrared Neural StimulationAuditory NeuronsNeural ExcitationNeuroscienzeElectrophysiologyLaser StimulationIn Vitro

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