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Measurement of Tension Release During Laser Induced Axon Lesion to Evaluate Axonal Adhesion to the Substrate at Piconewton and Millisecond Resolution
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Capitoli
- 00:05Titolo
- 01:27Laser Microsphere Manipulation
- 03:31UV and IR Beam Positioning and Optical Tweezer Calibration
- 04:56Axotomy and Simultaneous Force Measurement
- 06:50Results: Measurement of Changes in Cytoskeletal Equilibrium Caused by Laser Induced Lesions
- 08:50Conclusion
We measured the tension release in an axon that was partially lesioned with a laser dissector by simultaneous force spectroscopy measurement performed on an optically-trapped probe adhered to the membrane of the axon. The developed experimental protocol evaluates the axon adhesion to the culture substrate.
