È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 01:10Isolating Drosophila Ovaries
- 03:29Isolation of Mid-to-late Stage Drosophila Follicles
- 04:32In vitro Development of Stage 10B Follicles
- 06:43Applications for Isolated Mid-to-late Stage Drosophila Follicles
- 08:20Conclusion
Stage-specific isolation of mid-to-late Drosophila follicles is useful for a variety of purposes. Such follicles develop in culture, which allows for genetic and/or pharmacologic manipulations to be coupled with in vitro development assays and live imaging. Additionally, follicles can be used for molecular studies, such as isolating mRNA and protein.
