Isolation and Culture of Mouse Primary Pancreatic Acinar Cells

Johann Gout; Roxane M. Pommier; David F. Vincent; Bastien Kaniewski; Sylvie Martel; Ulrich Valcourt; Laurent Bartholin

doi:10.3791/50514

Rivista

/

Biologia

/

Isolation and Culture of Mouse Primary Pancreatic Acinar Cells

JoVE Journal
Biologia

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Biologia

Isolation and Culture of Mouse Primary Pancreatic Acinar Cells

DOI:

10.3791/50514-v

•

08:58 min

•

August 13, 2013

•

Johann Gout^2,3,4,5, Roxane M. Pommier^2,3,4,5, David F. Vincent^2,3,4,5, Bastien Kaniewski^2,3,4,5, Sylvie Martel^2,3,4,5, Ulrich Valcourt*^1,2,3,4,5, Laurent Bartholin*^1,2,3,4,5

¹INSERM U1052,Centre de Recherche en Cancérologie de Lyon, ²CNRS UMR5286,Centre de Recherche en Cancérologie de Lyon, ³Université de Lyon, ⁴Université Lyon 1, ⁵Centre Léon Bérard

Capitoli

00:05Titolo
01:42Pancreas Dissection and Dilaceration
03:51Enzymatic and Mechanical Dissociations of Pancreas
05:46Filtration, Seeding of Dispersed Acini, and Acinar Cell Culture
07:46Representative Acinar Culturing Results
08:23Conclusion

Summary

Traduzione automatica

In this publication, we describe a rapid and convenient procedure for isolating and culturing primary pancreatic acinar cells from the murine pancreas. This method constitutes a valuable approach to study the physiology of fresh primary normal/untransformed exocrine pancreatic cells.

Isolation and Culture of Mouse Primary Pancreatic Acinar Cells

•

•

•

Capitoli

Summary

Traduzione automatica

Tags

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Human Pancreatic Islet Isolation: Part II: Purification and Culture of Human Islets

Isolation of Primary Mouse Trophoblast Cells and Trophoblast Invasion Assay

Isolation and Primary Culture of Rat Hepatic Cells

Isolation of Immune Cells from Primary Tumors

Isolation and Culture of Neonatal Mouse Cardiomyocytes

Preparation of Pancreatic Acinar Cells for the Purpose of Calcium Imaging, Cell Injury Measurements, and Adenoviral Infection

Assessing the Secretory Capacity of Pancreatic Acinar Cells

Isolation and Primary Culture of Mouse Aortic Endothelial Cells

Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin

Isolation and Culture of Primary Aortic Endothelial Cells from Miniature Pigs

Read Article