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整装免疫荧光染色新生小鼠视网膜血管生成调查<em>在体内</em

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Whole Mount Immunofluorescent Staining of the Neonatal Mouse Retina to Investigate Angiogenesis In vivo

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整装免疫荧光染色新生小鼠视网膜血管生成调查<em>在体内</em

DOI:

10.3791/50546-v

•

08:47 min

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July 09, 2013

•

Simon Tual-Chalot, Kathleen R. Allinson, Marcus Fruttiger, Helen M. Arthur

¹Institute of Genetic Medicine,Newcastle University , ²UCL Institute of Ophthalmology,University College, London

Capitoli

00:05Titolo
00:56Enucleation and Fixation of Postnatal Eyes
02:01Dissection of Postnatal Murine Retinas
04:59Immuno/isolectin Staining of the Retinal Vasculature
07:07Typical Staining of Endothelial Cell and Pericytes
08:20Conclusion

Summary

Traduzione automatica

新生小鼠视网膜提供了一个很好的特点生理血管生成模型，它允许不同的基因或药物，在调节血管生成的角色的调查<em>在体内</em>上下文。免疫荧光染色准确地显示血管丛是这些类型的研究成功的关键。

Tags

Keywords: Angiogenesis Neonatal Mouse Retina Vascular Plexus Fluorescent Staining Isolectin Vascular Antibodies In Vivo Blood Vessel Formation 2D Structure Vascular Development Disease Modeling

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