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Renal Capsule Xenografting and Subcutaneous Pellet Implantation for the Evaluation of Prostate Carcinogenesis and Benign Prostatic Hyperplasia
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JoVE Journal Medicina
Renal Capsule Xenografting and Subcutaneous Pellet Implantation for the Evaluation of Prostate Carcinogenesis and Benign Prostatic Hyperplasia

Renal Capsule Xenografting and Subcutaneous Pellet Implantation for the Evaluation of Prostate Carcinogenesis and Benign Prostatic Hyperplasia

DOI:
10.3791/50574-v

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08:38 min

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August 28, 2013

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, , , , ,
1Department of Urology,University of Wisconsin-Madison, 2Medical Scientist (MD/PhD) Training Program,University of Rochester School of Medicine & Dentistry, 3Molecular and Environmental Toxicology Center,University of Wisconsin-Madison

Capitoli

  • 00:05Titolo
  • 01:44Preparation of Compressed Hormone Pellet
  • 02:57Preparation of Fire Polished Glass Pipette
  • 03:27Renal Capsule Xenografting
  • 06:02Subcutaneous Pellet Implantation
  • 06:35Results: Renal Capsule Grafting
  • 07:17Conclusion

Summary

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We describe the manufacture of compressed hormone pellets, as well as subcutaneous surgical implantation into mice. This strategy can be combined with the growth of cell and tissue xenografts under the renal capsule of athymic nude mice to evaluate hormonal carcinogenesis and regulation of benign prostate growth.

Tags

Prostate CarcinogenesisBenign Prostatic HyperplasiaSex Steroid HormonesAndrogenEstrogenSubcutaneous Pellet ImplantationRenal Capsule XenograftingHormone RegulationProstate Cell RecombinantsImmunocompromised Mice

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