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一个台式生物反应器的操作

JoVE Journal
Bioingegneria

This content is Free Access.

JoVE Journal Bioingegneria

Operation of a Benchtop Bioreactor

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一个台式生物反应器的操作

DOI:

10.3791/50582-v

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12:54 min

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September 12, 2013

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Kristina M. Obom, Andrew Magno, Patrick J. Cummings

¹Center for Biotechnology Education, Krieger School of Arts and Sciences,Johns Hopkins University, ²ATR Biotech

Capitoli

00:05Titolo
01:40Fermentor Vessel Set-up
02:31Fermentor Vessel Set-up: pH Probe Calibration
03:49Fermentor Vessel Set-up: Continuation of Vessel Set-up and Calibration of pO₂ Probe
08:01Fermentor Vessel Set-up: Inoculation and Sampling
10:03Results: A Typical Bioreactor Run
12:07Conclusion

Summary

Traduzione automatica

发酵罐是用来增加培养产量生物工程化细胞和生产率。在摇瓶筛选多种微生物或动物细胞培养人选后，下一个合乎逻辑的步骤是增加了选择的文化的生物质发酵罐。该视频演示了一个典型的台式生物反应器系统的设置和操作。

Tags

Fermentation System Cell Culture Bioreactor Temperature Control PH Control Dissolved Oxygen Fermentor Shake Flask Benchtop Fermentation Sterilization Inoculation Growth Medium Sampling Harvesting Data Analysis System Cleanup

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