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Genetisk kodede Molecular Probes at studere G-protein-koblede receptorer
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Biologia
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JoVE Journal Biologia
Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Genetisk kodede Molecular Probes at studere G-protein-koblede receptorer

DOI:
10.3791/50588-v

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16:16 min

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September 13, 2013

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, , , ,
1Laboratory of Chemical Biology and Signal Transduction,The Rockefeller University

Capitoli

  • 00:05Titolo
  • 02:23Site-specific Genetic Incorporation of Unnatural Amino Acids into GPCRs
  • 03:15Mapping a Ligand Binding Site Using Targeted Photocrosslinking
  • 07:12Targeted Epitope Tagging and Cell Surface Enzyme-linked Immunosorbent Assay (ELISA)
  • 10:23Bioorthogonal Fluorescent Labeling of a GPCR
  • 12:10Results: Versatile Applications of Site-specific azF Incorporation into GPCRs

Summary

Traduzione automatica

Traduzione automatica

Vi genetisk kode for den unaturlige aminosyre, p-azido-L-phenylalanin på forskellige målrettede positioner i GPCR'er og vise alsidigheden af ​​azidogruppen i forskellige applikationer. Heriblandt en målrettet fototværbinding teknologi til at identificere rester i den ligandbindende lomme af en GPCR, og stedspecifik bioorthogonal modificering af GPCR'er med et peptid-epitop-mærke eller fluorescerende probe.

Tags

Keywords: G Protein-coupled ReceptorsGenetically-encoded Molecular ProbesAmber Codon SuppressionP-azido-L-phenylalaninePhotocrosslinkingStaudinger-Bertozzi LigationPeptide-epitope TaggingFluorescent LabelingStructural And Dynamic Studies

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