Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue

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Biologia

JoVE Journal
Biologia

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JoVE Journal Biologia

Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue

DOI:

10.3791/50589-v

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09:20 min

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September 02, 2013

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Jacek R. Wiśniewski

¹Department of Proteomics and Signal Transduction,Max Planck Institute of Biochemistry

Capitoli

00:05Titolo
01:46Preparation of Tissue Slices for Microdissection and Sample Lysis
03:20Sample Processing
04:51Quantification of the Peptides
05:38Fractionation of the Lys-C and Tryptic Peptides
07:29Results: Peptide Quantification
08:21Conclusion

Summary

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Archival formalin fixed and paraffin embedded (FFPE) clinical samples are valuable material for investigation of diseases. Here we demonstrate a sample preparation workflow allowing in-depth proteomic analysis of microdissected FFPE tissue.

Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue

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