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Brug af Image Cytometry til kvantificering af patogene svampe i forbindelse med Host Cells
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells

Brug af Image Cytometry til kvantificering af patogene svampe i forbindelse med Host Cells

DOI:
10.3791/50599-v

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07:58 min

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June 19, 2013

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, , ,
1Department of Biology,Merrimack College, 2Center for Biotechnology and Biomedical Sciences,Merrimack College, 3Department of Technology R&D,Nexcelom Bioscience LLC

Capitoli

  • 00:05Titolo
  • 01:39Infection of Macrophages with H. capsulatum, C. albicans
  • 02:21Macrophage Lysis
  • 02:56CFU Plating
  • 03:28Visualization of Fungi within Live Macrophages
  • 03:54Image Cytometric Analysis
  • 06:04Results: H. capsulatum in Macrophage Cells
  • 07:12Conclusion

Summary

Traduzione automatica

Traduzione automatica

Her viser vi, hvordan billedet cytometri kan anvendes til kvantificering af patogene svampe i forbindelse med værtsceller i kultur. Denne teknik kan anvendes som et alternativ til CFU tælling.

Tags

Image CytometryPathogenic FungiCandida AlbicansHistoplasma CapsulatumCryptococcus NeoformansMacrophagesHost CellsColony Forming UnitFlow CytometryAcridine OrangePropidium IodideGFP-expressing Candida AlbicansQuantificationCellular PathogenesisInfection

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