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成比例的生物传感器测量活酵母细胞中的线粒体氧化还原状态和ATP
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JoVE Journal Bioingegneria
Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells
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成比例的生物传感器测量活酵母细胞中的线粒体氧化还原状态和ATP

DOI:
10.3791/50633-v

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12:22 min

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July 22, 2013

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, , , ,
1Department of Pathology and Cell Biology,Columbia University, 2Herbert Irving Comprehensive Cancer Center,Columbia University

Capitoli

  • 00:05Titolo
  • 02:48Growth of Cells and Preparation for Imaging
  • 03:29Mito-roGFP1 Imaging
  • 04:33MitGO-ATeam2 Imaging
  • 06:09ImageJ Analysis
  • 08:40Measuring Mitochondrial Redox State and ATP with Mito-roGFP1 and MitGO-ATeam2
  • 11:22Conclusion

Summary

Traduzione automatica

Traduzione automatica

我们描述了使用两个比例，基因编码生物传感器的，这是基于GFP，监测线粒体的氧化还原状态和细胞内ATP水平分辨率在活酵母细胞。

Tags

Mitochondrial Redox StateMitochondrial ATPRatiometric BiosensorsRoGFPMitGO-ATeamFRETYeast CellsEnergy MetabolismCalcium HomeostasisCellular LifespanProgrammed Cell Death

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