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Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers
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Capitoli
- 00:05Titolo
- 03:09Culturing a Cell Monolayer in Transwell Inserts
- 04:34Validation of the GI Epithelial Permeability Barrier using Transepithelial Electrical Resistance (TEER) and Immunostaining of Tight Junctions
- 06:29Evaluating Transepithelial Transport of Targeted Carriers
- 10:25Investigating the Mechanism of Transepithelial Transport of Targeted Nanocarriers
- 12:36Results: Caco-2 Cell Monolayer is a Model of Transepithelial Transport of Drug Nanocarriers (NCs) Across Cellular Barriers
- 17:50Conclusion
Many therapeutic applications require safe and efficient transport of drug carriers and their cargoes across cellular barriers in the body. This article describes an adaptation of established methods to evaluate the rate and mechanism of transport of drug nanocarriers (NCs) across cellular barriers, such as the gastrointestinal (GI) epithelium.
