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稳态，稳定前的状态，单营业额动力学测量DNA糖基化酶活性

JoVE Journal
Chimica

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JoVE Journal Chimica

Steady-state, Pre-steady-state, and Single-turnover Kinetic Measurement for DNA Glycosylase Activity

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稳态，稳定前的状态，单营业额动力学测量DNA糖基化酶活性

DOI:

10.3791/50695-v

•

14:27 min

•

August 19, 2013

•

Akira Sassa, William A. Beard, David D. Shock, Samuel H. Wilson

¹Laboratory of Structural Biology,NIEHS, National Institutes of Health

Capitoli

00:05Titolo
01:55Sample Preparation and Measurement of Steady-state Time Course and Active Site Titration of OGG1
05:53Measurement of Pre-steady-state Time Course
10:56Single-turnover Time Course
11:50Results: Kinetic Measurements of DNA Glycosylase Activity
14:01Conclusion

Summary

Traduzione automatica

时间8 - 羟基鸟嘌呤DNA糖苷酶糖基化酶活性课程双相参展产品的形成和爆发出的线性稳态阶段。利用淬火流技术中，突发和可以测量的稳态速率，分别对应到8 - 羟基鸟嘌呤和释放从产物DNA糖基化酶切除。

Tags

DNA Glycosylase OGG1 8-oxoG Steady-state Kinetics Pre-steady-state Kinetics Single-turnover Kinetics Burst Kinetics Product Release Enzyme Catalysis DNA Lesion Excision

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