Rivista
/
Neuroscienze
/
Fast Micro-iontophoresis of Glutamate and GABA: A Useful Tool to Investigate Synaptic Integration
JoVE Journal
Neuroscienze
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Neuroscienze
Fast Micro-iontophoresis of Glutamate and GABA: A Useful Tool to Investigate Synaptic Integration

Fast Micro-iontophoresis of Glutamate and GABA: A Useful Tool to Investigate Synaptic Integration

DOI:
10.3791/50701-v

•

07:08 min

•

July 31, 2013

•

,
1NRW Research Group ‘Dendritic integration in the CNS’, Department of Epileptology,University of Bonn, 2Neuronal Networks Group,Deutsches Zentrum für Neurodegenerative Erkrankungen e.V. (DZNE)

Capitoli

  • 00:05Titolo
  • 01:01Prepare and Test the Iontophoresis Pipettes
  • 02:38Generate a Postsynaptic Iontophoretic Potential
  • 04:56Representative Results for Fast Micro-iontophoresis of Glutamate and GABA
  • 06:45Conclusion

Summary

Traduzione automatica

Traduzione automatica

In this article we introduce fast micro-iontophoresis of neurotransmitters as a technique to investigate integration of postsynaptic signals with high spatial and temporal precision.

Tags

Micro-iontophoresisGlutamateGABASynaptic IntegrationDendritic TreeNeuronal OutputExcitatory InputsInhibitory InputsSpatial IntegrationTemporal IntegrationTwo-photon Scanning MicroscopeNeurotransmitter ReleasePresynapticPostsynaptic

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Using a Comparative Species Approach to Investigate the Neurobiology of Paternal Responses

Cut-loading: A Useful Tool for Examining the Extent of Gap Junction Tracer Coupling Between Retinal Neurons

MPI CyberMotion Simulator: Implementation of a Novel Motion Simulator to Investigate Multisensory Path Integration in Three Dimensions

Automated Quantification of Synaptic Fluorescence in C. elegans

Deriving the Time Course of Glutamate Clearance with a Deconvolution Analysis of Astrocytic Transporter Currents

Using an α-Bungarotoxin Binding Site Tag to Study GABA A Receptor Membrane Localization and Trafficking

Induction of an Isoelectric Brain State to Investigate the Impact of Endogenous Synaptic Activity on Neuronal Excitability In Vivo

Using Enzyme-based Biosensors to Measure Tonic and Phasic Glutamate in Alzheimer’s Mouse Models

Culturing In Vivo-like Murine Astrocytes Using the Fast, Simple, and Inexpensive AWESAM Protocol

Combining Optogenetics with Artificial microRNAs to Characterize the Effects of Gene Knockdown on Presynaptic Function within Intact Neuronal Circuits

Read Article