Rivista
/
Immunologia e infezioni
/
脂肪组织免疫细胞的分离
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Isolation of Adipose Tissue Immune Cells
Please note that all translations are automatically generated. Click here for the English version.

脂肪组织免疫细胞的分离

DOI:
10.3791/50707-v

•

07:09 min

•

May 22, 2013

•

, ,
1Department of Molecular Physiology and Biophysics,Vanderbilt University School of Medicine

Capitoli

  • 00:05Titolo
  • 01:11Harvesting and Preparation of Adipose Tissue
  • 02:06Collagenase Digestion
  • 03:19Staining of Cell Surface Antigens
  • 04:23FACS Analysis
  • 05:13Representative Results
  • 06:54Conclusion

Summary

Traduzione automatica

Traduzione automatica

脂肪组织（AT）是一个强烈的免疫细胞的激活和相互作用的部位。几乎所有的免疫系统的细胞是存在于AT和它们的比率是改变了肥胖。适当的隔离，定量，定性的AT免疫细胞群，是了解自己的角色在immunometabolic疾病的关键。

Tags

Immune CellsObesityMacrophagesT LymphocytesFlow CytometryStromal Vascular FractionImmunometabolism

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/50707?article_type=v

在固定和活细胞，流式细胞仪测量钙蛋白酶活性
check_url/it/50707?article_type=v

免疫细胞在受伤的脊髓组织流式细胞仪定量评估：一个细胞的分离纯化方法的新颖使用
check_url/it/50707?article_type=v

星状细胞的七个步骤
check_url/it/50707?article_type=v

流式细胞仪分析小鼠主动脉内流的免疫细胞
check_url/it/50707?article_type=v

心脏功能免疫细胞的分离
check_url/it/50707?article_type=v

活体成像的鼠标使用2 - 光子显微镜的胸腺
check_url/it/50707?article_type=v

主要鼠Ⅱ型肺泡上皮细胞的功能及分子生物学研究的流式细胞仪分离
check_url/it/50707?article_type=v

分离和流式细胞仪分析从缺血小鼠脑免疫细胞
check_url/it/50707?article_type=v

分离，鉴定和牙龈免疫细胞网络的功能性考试
check_url/it/50707?article_type=v

肾脏免疫细胞的可靠和高效率的提取

Read Article