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JoVE Journal
Immunology and Infection

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Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread
 

Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread

Article DOI: 10.3791/50723-v 15:31 min August 16th, 2013
August 16th, 2013

Capitoli

Riepilogo

Live cell imaging of alphaherpes virus infections enables analysis of the dynamic events of directed transport and intercellular spread. Here, we present methodologies that utilize recombinant viral strains expressing fluorescent fusion proteins to facilitate visualization of viral assemblies during infection of primary neurons.

Tags

Anterograde Transport Live Cell Imaging Fluorescent Fusion Proteins Viral Particle Assemblies Cell-cell Spread Particle Transport Velocities Protein Localization Viral Genetic Techniques Alphaherpes Virus
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