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的代谢活性细菌在通才的肠道鉴定<em>海灰翅夜蛾</em>通过DNA的稳定同位素探测使用<sup> 13</sup> C-葡萄糖

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Biologia

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JoVE Journal Biologia

Identification of Metabolically Active Bacteria in the Gut of the Generalist Spodoptera littoralis via DNA Stable Isotope Probing Using ¹³C-Glucose

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的代谢活性细菌在通才的肠道鉴定<em>海灰翅夜蛾</em>通过DNA的稳定同位素探测使用<sup> 13</sup> C-葡萄糖

DOI:

10.3791/50734-v

•

12:11 min

•

November 13, 2013

•

Yongqi Shao*¹, Erika M Arias-Cordero*¹, Wilhelm Boland

¹Department of Bioorganic Chemistry,Max Planck Institute for Chemical Ecology

Capitoli

00:05Titolo
02:35¹³C-Labeling of DNA in Metabolically Active Gut Bacteria
03:42Insect Dissection
05:06DNA Extraction and Amplification
06:13CsCl Gradient/DNA Ultracentrifugation
08:27Processing the CsCl Gradients
09:45Identifying Metabolically Active Gut Bacteria
11:13Conclusion

Summary

Traduzione automatica

与海灰翅夜蛾的肠道相关活动的细菌群落，由稳定同位素探测（SIP）耦合到焦磷酸测序确定。使用这种方法，识别社区内的代谢活性细菌种类与高的分辨率和精度完成。

Tags

Stable Isotope Probing 13C-glucose Metabolically Active Bacteria Spodoptera Littoralis Insect Gut Microbial Community Pyrosequencing Culture-independent Techniques DNA Density Gradient Centrifugation

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