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Mikroinjektion Wound Assay og<em> In vivo</em> Lokalisering af epidermale Wound Respons Journalister i<em> Drosophila</em> Embryoner.
JoVE Journal
Bioingegneria
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JoVE Journal Bioingegneria
Microinjection Wound Assay and In vivo Localization of Epidermal Wound Response Reporters in Drosophila Embryos.

Mikroinjektion Wound Assay og<em> In vivo</em> Lokalisering af epidermale Wound Respons Journalister i<em> Drosophila</em> Embryoner.

DOI:
10.3791/50750-v

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11:12 min

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November 01, 2013

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, , ,
1Sophie Davis School of Biomedical Education,The City College of New York, 2Cell & Developmental Biology,University of California, San Diego

Capitoli

  • 00:05Titolo
  • 01:38Embryo Collection and Preparation
  • 04:33Embryo Wounding
  • 06:18Embryo Microinjection
  • 07:34Formaldehyde Fixation Notes
  • 09:00Gene Expression at Wound Sites
  • 10:45Conclusion

Summary

Traduzione automatica

Traduzione automatica

De embryonale epidermis af meget sent Drosophila embryoner giver et in vivo system for hurtig analyse stiksår respons og kan kombineres med genetiske manipulationer eller kemiske mikroinjektion behandlinger til at fremme studier i sårheling til oversættelse til pattedyr modeller.

Tags

DrosophilaEmbryoEpidermalWound ResponseMicroinjectionPuncture InjuryWound Transcriptional ReportersHemocyte RecruitmentWound HealingRegenerative ResponseInnate Immune Response

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