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Protokollerne til gennemførelse af en<em> Escherichia coli</em> Baseret TX-TL Cell-Free Expression System for syntetisk biologi
JoVE Journal
Biologia
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JoVE Journal Biologia
Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

Protokollerne til gennemførelse af en<em> Escherichia coli</em> Baseret TX-TL Cell-Free Expression System for syntetisk biologi

DOI:
10.3791/50762-v

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16:11 min

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September 16, 2013

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, , , , ,
1Department of Biology,California Institute of Technology, 2Department of Bioengineering,California Institute of Technology, 3Synthetic Biology Center, Department of Bioengineering,Massachusetts Institute of Technology, 4School of Physics and Astronomy,University of Minnesota

Capitoli

  • 00:05Titolo
  • 01:41Cell Lysis
  • 06:29Extract Clarification
  • 08:32Experimental Execution of a TX-TL Reaction
  • 10:40Results: Assessment of TX-TL Cell-free Expression System
  • 15:56Conclusion

Summary

Traduzione automatica

Traduzione automatica

Denne fem-dages-protokollen skitserer alle trin, udstyr og supplerende software er nødvendig for at skabe og drive en effektiv endogent Escherichia coli baserede TX-TL cellefrit udtryk system fra bunden. Med reagenser, protokollen tager 8 timer eller mindre til opsætning af en reaktions, indsamle og behandle data.

Tags

Cell-free Expression SystemEscherichia ColiTX-TLTranscription-translationSynthetic BiologyProtein ExpressionIn VitroEndogenousT7 RNA PolymeraseLac/tet RepressorsMRNA DegradationDNA DegradationBiomolecular Breadboard

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