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Isolering og Th17 Differentiering af Naïve CD4 T-lymfocytter
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Isolering og Th17 Differentiering af Naïve CD4 T-lymfocytter

DOI:
10.3791/50765-v

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12:59 min

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September 26, 2013

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, , , ,
1Department of Microbiology and Cell Science,The University of Florida

Capitoli

  • 00:05Titolo
  • 01:27Plate-bound anti-CD3
  • 02:03Mouse Dissection
  • 04:40Th17 Differentiation
  • 05:40qPCR and ELISA
  • 06:27Cell Activation
  • 07:09Intracellular Staining
  • 08:55Flow Cytometric Analysis
  • 09:24Results: Isolation and Differentiation of Th17 Cells
  • 12:14Conclusion

Summary

Traduzione automatica

Traduzione automatica

Med effektorfunktioner adskiller sig fra andre T-celle delmængder, har Th17 celler blevet centralt impliceret i inflammatoriske autoimmunitet. Denne in vitro Th17 differentiering protokol giver et middel til at afgøre, om naive CD4 + T-lymfocytter kan differentiere sig Th17 celler, og for yderligere at undersøge deres rolle i autoimmunitet og vært reaktion.

Tags

Keywords: Th17 CellsCD4+ T LymphocytesAutoimmune DisordersIL-17T Cell DifferentiationFlow CytometryQPCRELISA

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