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Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications
 

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Article DOI: 10.3791/50772-v • 14:43 min • September 23rd, 2013
September 23rd, 2013 •
1, 1, 1, 1, 1, 1, 1, 1
1Center for Systems Biology, Massachusetts General Hospital

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We present a method for rapid, reversible immobilization of small molecules and functionalized nanoparticle assemblies for Surface Plasmon Resonance (SPR) studies, using sequential on-chip bioorthogonal cycloaddition chemistry and antibody-antigen capture.

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Keywords: Microfluidic On-chip Capture-cycloaddition Reversible Immobilization Small Molecules Multi-component Structures Biosensor Applications Trans-cyclooctene Tetrazine Surface Plasmon Resonance Non-covalent Immobilization Antigen-antibody AP1497 FKBP12 Nanoparticle
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