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A Rapid Protocol for Integrating Extrachromosomal Arrays With High Transmission Rate into the C. elegans Genome
 
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A Rapid Protocol for Integrating Extrachromosomal Arrays With High Transmission Rate into the C. elegans Genome

Article DOI: 10.3791/50773-v • 06:33 min • December 9th, 2013
December 9th, 2013 •
1,2, 1,2, 1,2, 1,2
1Université Claude Bernard Lyon, 2Centre de Génétique et de Physiologie Moléculaires et Cellulaires, CNRS UMR 5534

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This protocol describes a rapid and low material consuming procedure for the integration of transgenic extrachromosomal arrays into the Caenorhabditis elegans genome using ultra violet (UV) irradiation. Furthermore, this protocol is particularly well suited for transgenic lines that transmit extrachromosomal arrays at a high rate.

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Keywords: C. Elegans Extrachromosomal Arrays Transgenic Lines UV Irradiation Genome Integration High Transmission Rate Mendelian Segregation Nonmosaic Expression Rapid Protocol Transgene Expression
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