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Immunologia e infezioni

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培养和维护<em>难辨梭状芽孢杆菌</em>在厌氧环境

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Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Culturing and Maintaining Clostridium difficile in an Anaerobic Environment

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培养和维护<em>难辨梭状芽孢杆菌</em>在厌氧环境

DOI:

10.3791/50787-v

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11:13 min

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September 14, 2013

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Adrianne N. Edwards, Jose M. Suárez, Shonna M. McBride

¹Department of Microbiology and Immunology,Emory University School of Medicine

Capitoli

00:05Titolo
01:51Culturing, Enumerating, and Storing C. difficile from Stool Samples
05:59Culturing C. difficile from Frozen Glycerol Stocks
07:10Purifying Spores from C. difficile
09:12Results: C. difficile Successfully Grown on Various Media
10:16Conclusion

Summary

Traduzione automatica

艰难梭菌是一种致病细菌是严格厌氧并导致抗生素相关性腹泻（AAD）。这里，用于分离，培养和维持C.方法艰难梭菌营养细胞和孢子的描述。这些技术的必要厌氧室，这就需要定期保养，以确保最佳的C。适当的条件难辨栽培。

Tags

Keywords: Clostridium Difficile Anaerobic Environment Anaerobic Chamber Fecal Samples Isolation Cultivation Glycerol Stocks Spore Stocks Antibiotic-associated Diarrhea Nosocomial Pathogen

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