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Cell的传播分析单细胞显微注射
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Biologia
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JoVE Journal Biologia
Single-cell Microinjection for Cell Communication Analysis
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Cell的传播分析单细胞显微注射

DOI:
10.3791/50836-v

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09:59 min

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February 26, 2017

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, , , ,
1Institute Oswaldo Cruz, Laboratory of Cellular Communication,Oswaldo Cruz Foundation

Capitoli

  • 00:00Titolo
  • 00:50The Components
  • 01:47Preparing the Pipette
  • 06:17Results: A Real Experiment
  • 09:27Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里，我们将介绍如何执行路西法黄色的单细胞显微注射可视化在活细胞通过缝隙连接处蜂窝通信，并提供一些有用的提示。我们希望本文可以帮助大家来评估细胞耦合程度由于功能间隙连接。这里所描述的一切可能的，在原则上，适合于与低于1,000道尔顿分子量其它荧光染料。

Tags

Single-cell MicroinjectionCell Communication AnalysisP2 ReceptorsGap JunctionsImmune SystemLiverMicroinjection SetupFluorescence MicroscopeCCD CameraCurrent GeneratorMicroelectrodeMicro-manipulatorBorosilicate Glass CapillaryPolarTungsten FilamentDye LoadingSodium SolutionSilver WireHead StagePetri DishMagnification LensCell TargetingMicroelectrode Testing

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