High Throughput Microinjections of Sea Urchin Zygotes

Nadezda A. Stepicheva; Jia L. Song

doi:10.3791/50841

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Biologia

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High Throughput Microinjections af søpindsvin Zygoter

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Biologia

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JoVE Journal Biologia

High Throughput Microinjections of Sea Urchin Zygotes

High Throughput Microinjections af søpindsvin Zygoter

DOI:

10.3791/50841-v

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12:40 min

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January 21, 2014

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Nadezda A. Stepicheva, Jia L. Song

¹Department of Biological Sciences,University of Delaware

Capitoli

00:05Titolo
01:26Experimental Preparations
04:00Collecting Sea Urchin Gametes
06:12Rowing the Eggs
07:40Microinjection of the Sea Urchin Zygotes
11:13Injection of Reporter Constructs Does Not Affect Development
12:05Conclusion

Summary

Traduzione automatica

Mikroinjektion er en almindelig teknik, der anvendes til at levere DNA-konstruktioner, mRNA'er, morpholino antisense-oligonucleotider eller andre behandlinger i æg, embryoner, og celler fra forskellige arter.

High Throughput Microinjections af søpindsvin Zygoter

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Summary

Traduzione automatica

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