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Isolation, Culture, and Transplantation of Muscle Satellite Cells
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JoVE Journal Biologia
Isolation, Culture, and Transplantation of Muscle Satellite Cells

Isolation, Culture, and Transplantation of Muscle Satellite Cells

DOI:
10.3791/50846-v

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10:25 min

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April 08, 2014

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, ,
1Stem Cell Institute, Paul and Sheila Wellstone Muscular Dystrophy Center, Department of Neurology,University of Minnesota Medical School

Capitoli

  • 00:05Titolo
  • 02:07Isolation of Mononuclear Cells from Mouse Skeletal Muscle
  • 04:19Antibody Staining and Separation with MACS
  • 06:39Myoblast Differentiation and Transplantation for Skeletal Muscle Regeneration
  • 08:50Results: Representative Muscle Satellite Cell Transplantation
  • 09:52Conclusion

Summary

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The isolation and culture of a pure population of quiescent satellite cells, a muscle stem cell population, is essential to the understanding of muscle stem cell biology and regeneration, as well as stem cell transplantation for therapies in muscular dystrophy and other degenerative diseases. 

Tags

Keywords: Muscle Satellite CellsSkeletal MuscleRegenerationMyoblast TransplantationMuscle Stem CellsCell IsolationMACSFACSMuscular DystrophyCell Therapy

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