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Berigelse og udrensning af humane embryonale stamceller ved påvisning af celleoverfladeantigener ved hjælp af monoklonale antistoffer TG30 og GCTM-2
JoVE Journal
Biologia
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JoVE Journal Biologia
Enrichment and Purging of Human Embryonic Stem Cells by Detection of Cell Surface Antigens Using the Monoclonal Antibodies TG30 and GCTM-2

Berigelse og udrensning af humane embryonale stamceller ved påvisning af celleoverfladeantigener ved hjælp af monoklonale antistoffer TG30 og GCTM-2

DOI:
10.3791/50856-v

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12:43 min

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December 06, 2013

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, , , , ,
1Materials Science and Engineering,CSIRO

Capitoli

  • 00:05Titolo
  • 01:38Dissociation of Human Embryonic Stem Cells (hESC) Bulk Cultures into Single Cells
  • 02:44Immunofluorescent Staining of Cell Surface Antigens TG30 and GCTM-2
  • 05:02Post-FACS Culture of TG30/GCTM-2 Cell Subfractions
  • 07:46Enrichment of Pluripotent hESC from TG30Hi-GCTM-2Hi Cells
  • 11:28Conclusion

Summary

Traduzione automatica

Traduzione automatica

Vi beskriver brugen af de monoklonale antistoffer TG30 (CD9) og GCTM-2 til kombineret påvisning af celleoverfladeantigener via fluorescensaktiveret cellesortering (FACS) til identifikation og berigelse af levende menneskelige embryonale stamceller (hESC) ved hjælp af positiv udvælgelse og også brugen af negativ udvælgelse til at rense HESC'er fra en blandet cellepopulation.

Tags

Human Embryonic Stem CellsHESCCell Surface AntigensTG30GCTM-2FACSPluripotencyDifferentiationPurgingEnrichmentTeratomaTransplantation

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