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Capitoli
- 00:05Titolo
- 01:33Prepare and Screen Co-culture Plates
- 03:20Isolate Putative Inducing Organisms and Streak for Isolated Single Colonies
- 04:54Retest in Secondary Screen to Confirm Hits
- 05:54Representative Microbial Interaction Results
- 07:59Conclusion
Bacteria produce secreted compounds that have the potential to affect the physiology of their microbial neighbors. Here we describe a coculture screen that allows detection of such chemically mediated interspecies interactions by mixing soil microbes with fluorescent transcriptional reporter strains of Bacillus subtilis on solid media.
