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Capitoli
- 00:05Titolo
- 01:43Bladder Cancer Induction and Vaccination Treatments
- 02:49Monitoring and Sample Collection
- 04:11Multiplex Fluorometric Microbead Immunoassay
- 06:37IFN-γ/IL-4 ELISpot Preparation and Analysis
- 08:07Representative Transitional Cell Bladder Carcinoma Induction Results
- 10:39Conclusion
An N-butyl-N-(4-hydroxybutyl)nitrosamine-induced bladder cancer model was developed in human mucin 1 (MUC1) transgenic mice for the purpose of testing MUC1-directed immunotherapy. After administering a MUC1-targeted peptide vaccine, a cytotoxic T lymphocyte response to MUC1 was confirmed by measuring serum cytokine levels and T-cell specific activity.
