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Novel Whole-tissue Quantitative Assay of Nitric Oxide Levels in Drosophila Neuroinflammatory Response
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Capitoli
- 00:05Titolo
- 01:18Dissecting Adult Fly Brains and Incubation
- 02:23Preparation During Incubation
- 02:53Detection of Nitrite Levels
- 04:11Results: NOS and Paraquat Toxicity
- 07:16Conclusion
Levels of the inflammatory cell-signaling molecule nitric oxide (NO) are commonly assayed using Griess reagent. In this protocol, we have created a modified Griess assay utilizing live Drosophila brain tissue in order to detect the secretion of NO in a simple, quantifiable and highly repeatable method.
