È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
1Department of Surgery, Davis Heart and Lung Research Institute,The Ohio State University Wexner Medical Center, 2Department of Physiology and Cell Biology, Davis Heart and Lung Research Institute,The Ohio State University Wexner Medical Center, 3Department of Molecular Biophysics and Physiology,Rush University Medical Center, 4Department of Internal Medicine, Davis Heart and Lung Research Institute,The Ohio State University Wexner Medical Center
Capitoli
- 00:05Titolo
- 01:29Setting Up Osmotic-stress Perfusion System and Solution Preparation
- 02:00Preparing Intact Single Flexor Digitorum Brevis (FDB) Muscle Fibers from Mouse
- 05:12Fluo-4 AM Dye Loading and Ca2+ Imaging (Sparks Measurement)
- 07:39Data Analysis
- 08:14Results: Ca2+ Sparks in Young, Aged, and Diseased Mice
- 10:28Conclusion
Beskrevet her er en metode til direkte at måle calcium gnister, de elementære enheder af Ca 2 + frigivelse fra sarkoplasmiske reticulum i intakte skeletmuskulatur fibre. Denne metode udnytter osmotisk stress-medieret udløsning af Ca 2 + frigivelse fra ryanodine receptor i isolerede muskelfibre. Dynamik og homeostatiske kapacitet af intracellulær Ca 2 +-signalering, kan anvendes til at vurdere muskelfunktionen hos sundhed og sygdom.
