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1Department of Surgery, Davis Heart and Lung Research Institute,The Ohio State University Wexner Medical Center, 2Department of Physiology and Cell Biology, Davis Heart and Lung Research Institute,The Ohio State University Wexner Medical Center, 3Department of Molecular Biophysics and Physiology,Rush University Medical Center, 4Department of Internal Medicine, Davis Heart and Lung Research Institute,The Ohio State University Wexner Medical Center
Capitoli
- 00:05Titolo
- 01:29Setting Up Osmotic-stress Perfusion System and Solution Preparation
- 02:00Preparing Intact Single Flexor Digitorum Brevis (FDB) Muscle Fibers from Mouse
- 05:12Fluo-4 AM Dye Loading and Ca2+ Imaging (Sparks Measurement)
- 07:39Data Analysis
- 08:14Results: Ca2+ Sparks in Young, Aged, and Diseased Mice
- 10:28Conclusion
Hier beschreven is een methode om direct te meten calcium vonken, de elementaire eenheden van Ca2 + vrij van sarcoplasmatisch reticulum in intacte spiervezels. Deze methode maakt gebruik van osmotische stress-gemedieerde activering van Ca 2 + vrijlating uit ryanodinereceptor in geïsoleerde spiervezels. De dynamiek en homeostatische aantal intracellulaire Ca2 +-signalen kunnen worden gebruikt om spierfunctie in gezondheid en ziekte te beoordelen.
