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Mus Genome Engineering Brug Designer Nucleaser
JoVE Journal
Biologia
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JoVE Journal Biologia
Mouse Genome Engineering Using Designer Nucleases

Mus Genome Engineering Brug Designer Nucleaser

DOI:
10.3791/50930-v

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12:04 min

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April 02, 2014

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, , ,
1Institute of Laboratory Animal Science,University of Zurich, 2Department of Genetics, Cell Biology & Development and Center for Genome Engineering,University of Minnesota

Capitoli

  • 00:05Titolo
  • 01:18Identifying TALEN Target Sites
  • 02:30Embryo Isolation
  • 04:23Embryo Microinjection
  • 06:33Embryo Transfer
  • 09:15Identifying Founders with Targeted Mutations
  • 11:21Conclusion

Summary

Traduzione automatica

Traduzione automatica

Designer nucleaser såsom zinkfinger nukleaser (ZFNs) og transskription aktivator-lignende effektor nucleaser (TALENS) kan anvendes til at modificere genomet af muse præimplantations embryoner ved at udløse både homolog ende sammenføjning (NHEJ), og homolog rekombination (HR) veje. Disse fremskridt gør det muligt hurtig generering af mus med præcise genetiske modifikationer.

Tags

Mouse Genome EngineeringDesigner NucleasesZinc Finger Nucleases (ZFNs)Transcription Activator-like Effector Nucleases (TALENs)CRISPR/Cas9Site-specific Genome ModificationKnockoutKnock-inTransgenic MiceGenome EditingMicroinjectionFertilized Mouse Oocytes

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