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MACS纯化光感受器前体细胞的亚视移植到成年小鼠视网膜中

JoVE Journal
Medicina

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JoVE Journal Medicina

Subretinal Transplantation of MACS Purified Photoreceptor Precursor Cells into the Adult Mouse Retina

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MACS纯化光感受器前体细胞的亚视移植到成年小鼠视网膜中

DOI:

10.3791/50932-v

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08:28 min

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February 22, 2014

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Dominic Eberle*¹, Tiago Santos-Ferreira*¹, Sandra Grahl, Marius Ader

¹CRTD / DFG-Research Center for Regenerative Therapies Dresden,Technische Universität Dresden

Capitoli

00:05Titolo
01:33Retina Dissociation
03:21Cell Sorting Using Magnetic Associated Cell Sorting (MACS)
05:07Transplantation of MAC-sorted Photoreceptor Precursor Cells Into the Mouse Retina
06:39Results: Donor GFP+ Rod Photoreceptor Integration Into the Host Retina
07:42Conclusion

Summary

Traduzione automatica

细胞移植是治疗视网膜退化的一种策略，其特征是光感受器丧失。在这里，我们描述了一种丰富可移植光感受器及其亚视移植到成年小鼠的方法。

Tags

Keywords: Subretinal Transplantation Photoreceptor Precursor Cells MACS Adult Mouse Retina Vision Impairment Blindness Photoreceptor Replacement Cell Integration Cell Sorting CD73 Rod Photoreceptor Precursors

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