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电池挤压作为一种强大的，微流控细胞内传送平台

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Bioingegneria

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JoVE Journal Bioingegneria

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

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电池挤压作为一种强大的，微流控细胞内传送平台

DOI:

10.3791/50980-v

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08:02 min

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November 07, 2013

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Armon Sharei, Nahyun Cho, Shirley Mao, Emily Jackson, Roberta Poceviciute, Andrea Adamo, Janet Zoldan, Robert Langer², Klavs F Jensen

¹Department of Chemical Engineering,Massachusetts Institute of Technology, ²David H. Koch Institute for Integrative Cancer Research,Massachusetts Institute of Technology

Capitoli

00:05Titolo
01:20Experiment Preparation
02:19Assembly of the Intracellular Delivery Platform
03:28Cell Preparation and Device Operation
06:13Results: Delivery of Dextran into HeLa Cells
07:21Conclusion

Summary

Traduzione automatica

细胞的快速机械变形已成为细胞内输送大分子和纳米材料的一种很有前途的，矢量-free方法。这个协议提供了关于如何使用该系统进行范围广泛的应用中的详细步骤。

Tags

Cell Squeezing Intracellular Delivery Microfluidic Device Vector-free Delivery Cell Membrane Disruption Primary Immune Cells Cell Reprogramming Carbon Nanotubes Quantum Dots Optimization Device Assembly Cell Preparation System Operation

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