È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 01:38Dissection of Wholemount Retina
- 02:50Mounting onto Hydrophilized PTFE Membrane Insert
- 03:43Bolus Injection of Isolectin Alexa 488
- 05:05Vertical Sectioning of Membrane-mounted Tissue
- 06:45Results: Hydrophilized PTFE Membrane for Wholemount Retina
- 08:04Conclusion
The protocol described here is for structural assessment of a wholemount retinal preparation. This includes descriptions of tissue dissection, mounting onto a hydrophilized polytetrafluoroethylene (PTFE) membrane insert, bolus loading with fluorescent markers, and a comparison of fixation with carbodiimide and paraformaldehyde for immunohistochemical analysis of cellular and synaptic components.
