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The Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Technique With Fluorometry
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JoVE Journal Biologia
The Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Technique With Fluorometry

The Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Technique With Fluorometry

DOI:
10.3791/51040-v

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10:57 min

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March 11, 2014

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, , , ,
1Department of Biomedical Engineering,Washington University in St. Louis

Capitoli

  • 00:05Titolo
  • 01:32Agar Bridge and Cut-open Rig Preparation
  • 03:54Cut-open Procedure
  • 09:48Results: The Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Technique with Fluorometry
  • 10:27Conclusion

Summary

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The cut-open Vaseline gap approach is used to obtain low noise recordings of ionic and gating currents from voltage-dependent ion channels expressed in Xenopus oocytes with high resolution of fast channel kinetics. With minor modification, voltage clamp fluorometry can be coupled to the cut-open oocyte protocol.

Tags

Keywords: Xenopus OocyteCut-open Vaseline GapVoltage-clampFluorometryElectrophysiologyIon ChannelsHNaV1.5Two-electrode Voltage ClampFast KineticsVoltage Clamp Fluorometry

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