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Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes
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JoVE Journal Immunologia e infezioni
Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes

Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes

DOI:
10.3791/51043-v

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14:05 min

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September 19, 2013

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, , , , , ,
1Unité des Interactions Bactéries Cellules,Pasteur Institute, 2INSERM U604, 3Institut National de la Recherche Agronomique (INRA), USC2020, 4Institute of Biochemistry,ETH Zürich, 5Focal Area Infection Biology, Biozentrum,University of Basel

Capitoli

  • 00:05Titolo
  • 01:39Reverse siRNA Cell Transfection
  • 04:14Cellular Infection
  • 06:57Staining for InlC Protein
  • 09:19Results: Cell Infection by L. monocytogenes is Detected by Fluorescent Labeling of Cytoplasmic InlC
  • 13:27Conclusion

Summary

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Listeria monocytogenes is a Gram positive bacterial pathogen frequently used as a major model for the study of intracellular parasitism. Imaging late L. monocytogenes infection stages within the context of small-interfering RNA screens allows for the global study of cellular pathways required for bacterial infection of target host cells.

Tags

Listeria MonocytogenesBacterial PathogenIntracellular ParasitismCellular Immune ResponseInlCFluorescent LabelingMicroscopyHigh-throughput SiRNA ScreeningCellular Signaling Pathways

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